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PubMed · 11892998

Mouse beta-mannosidase: cDNA cloning, expression, and chromosomal localization.

T Beccari·L Bibi·S Stinchi·J L Stirling·A Orlacchio
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Abstract

Beta-mannosidase is an exoglycosidase involved in the degradation of N-linked oligosaccharides moieties of glycoproteins. Lack of beta-mannosidase activity leads to the lysosomal disorder beta-mannosidosis (MIM 248510). We have isolated and sequenced the gene encoding the mouse beta-mannosidase. Comparison of the deduced amino acid sequence of mouse, human, bovine, and goat beta-mannosidase showed 64%, identity, reflecting a high degree of evolutionary conservation. Analysis of a multiple tissue northern blotting revealed a major transcript of about 3.7 kb in all tissues examined. The northern analysis also demonstrates that there is differential tissue mRNA expression. The mouse beta-mannosidase gene (Bmn) was mapped to the distal end of Chromosome (Chr) 3, in a region that is homologous with a segment of human Chr 4 containing the orthologous human gene.

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AnimalsCattleCell NucleusChromosome MappingChromosomesChromosomes, Human, Pair 4Cloning, MolecularDNA, ComplementaryEukaryotic CellsEvolution, MolecularGene Expression Regulation, EnzymologicGoatsHumansLysosomal Storage DiseasesLysosomesMannosidasesMiceMolecular Sequence DataPhylogenyRNA, MessengerSequence Homology, Amino AcidSequence Homology, Nucleic AcidViscerabeta-MannosidaseComparative StudyJournal ArticleResearch Support, Non-U.S. Gov't

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T Beccari, L Bibi, S Stinchi, J L Stirling, A Orlacchio. 2001. Mouse beta-mannosidase: cDNA cloning, expression, and chromosomal localization.. https://doi.org/10.1023/a%3A1013286216030

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