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PubMed · 11901840

PD-loop technology: PNA openers at work.

V V Demidov
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Abstract

A concise survey of the emerging PD-loop technology is presented, which outlines several exemplary methods with robust DNA diagnostic potential: duplex DNA capture, topological DNA labeling, nondenaturing DNA sequencing and hybridization of molecular beacons to double-stranded DNA. Advantages of these new PNA-based assays over existing techniques for sequence-specific detection and manipulation of DNA duplexes are discussed. Future prospects for the further development of PD-loop technology are highlighted.

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Base SequenceDNADNA, ComplementaryHumansModels, ChemicalModels, GeneticMolecular Diagnostic TechniquesMolecular Sequence DataNucleic Acid ConformationSequence Analysis, DNAJournal ArticleResearch Support, U.S. Gov't, P.H.S.

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V V Demidov. 2001. PD-loop technology: PNA openers at work.. https://doi.org/10.1586/14737159.1.3.343

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