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Hepatitis C virus-like particle morphogenesis.

Emmanuelle Blanchard·Denys Brand·Sylvie Trassard·Alain Goudeau·Philippe Roingeard
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Abstract

Although much is known about the hepatitis C virus (HCV) genome, first cloned in 1989, little is known about HCV structure and assembly due to the lack of an efficient in vitro culture system for HCV. Using a recombinant Semliki forest virus replicon expressing genes encoding HCV structural proteins, we observed for the first time the assembly of these proteins into HCV-like particles in mammalian cells. This system opens up new possibilities for the investigation of viral morphogenesis and virus-host cell interactions.

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AnimalsCell LineCricetinaeHepacivirusHumansMicroscopy, ElectronMorphogenesisRepliconSemliki forest virusViral Structural ProteinsVirionVirus AssemblyJournal ArticleResearch Support, Non-U.S. Gov't

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Emmanuelle Blanchard, Denys Brand, Sylvie Trassard, Alain Goudeau, Philippe Roingeard. 2002. Hepatitis C virus-like particle morphogenesis.. https://doi.org/10.1128/jvi.76.8.4073-4079.2002

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