Scaffold/matrix attachment region elements interact with a p300-scaffold attachment factor A complex and are bound by acetylated nucleosomes.

Joost H A Martens; Matty Verlaan; Eric Kalkhoven; Josephine C Dorsman; Alt Zantema

doi:10.1128/mcb.22.8.2598-2606.2002

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PubMed · 11909954

Scaffold/matrix attachment region elements interact with a p300-scaffold attachment factor A complex and are bound by acetylated nucleosomes.

Joost H A Martens·Matty Verlaan·Eric Kalkhoven·Josephine C Dorsman·Alt Zantema

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Abstract

The transcriptional coactivator p300 regulates transcription by binding to proteins involved in transcription and by acetylating histones and other proteins. These transcriptional effects are mainly at promoter and enhancer elements. Regulation of transcription also occurs through scaffold/matrix attachment regions (S/MARs), the chromatin regions that bind the nuclear matrix. Here we show that p300 binds to the S/MAR binding protein scaffold attachment factor A (SAF-A), a major constituent of the nuclear matrix. Using chromatin immunoprecipitations, we established that both p300 and SAF-A bind to S/MAR elements in the transiently silent topoisomerase I gene prior to its activation at G(1) during cell cycle. This binding is accompanied by local acetylation of nucleosomes, suggesting that p300-SAF-A interactions at S/MAR elements of nontranscribed genes might poise these genes for transcription.

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Acetylation Adenovirus E1A Proteins Cell Cycle Heterogeneous-Nuclear Ribonucleoprotein U Heterogeneous-Nuclear Ribonucleoproteins Humans In Vitro Techniques Leukemia, Plasma Cell Macromolecular Substances Nuclear Proteins Nucleosomes Protein Binding Recombinant Proteins Ribonucleoproteins Trans-Activators Transcription, Genetic Two-Hybrid System Techniques Journal Article Research Support, Non-U.S. Gov't

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Joost H A Martens, Matty Verlaan, Eric Kalkhoven, Josephine C Dorsman, Alt Zantema. 2002. Scaffold/matrix attachment region elements interact with a p300-scaffold attachment factor A complex and are bound by acetylated nucleosomes.. https://doi.org/10.1128/mcb.22.8.2598-2606.2002

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