Sampling wheat for deoxynivalenol.

Thomas B Whitaker; Winston M Hagler; Francis G Giesbrecht; Anders S Johansson

doi:10.1007/978-1-4615-0629-4_8

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Sampling wheat for deoxynivalenol.

Thomas B Whitaker·Winston M Hagler·Francis G Giesbrecht·Anders S Johansson

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Abstract

The variability associated with testing wheat for deoxynivalenol (DON) was measured using a 0.454 kg sample, a Romer mill, 25 g of comminuted subsample and the Romer Fluoroquant analytical method. The total variability was partitioned into sampling, sample preparation, and analytical variability components. Each variance component was found to be a function of the DON concentration and equations were developed to predict each variance component using regression techniques. The effects of sample size, subsample size, and number of aliquots on reducing the variability of the DON test procedure were also determined. Using the test procedure described above, the coefficient of variation (CV) associated with testing wheat at 5 ppm DON was found to be 13.4%. The CVs associated with sampling, sample preparation, and analysis were 6.3, 10.0, and 6.3%, respectively. The sample variations associated with testing wheat are relatively small when compared to CVs associated with testing other commodities for other mycotoxins such as aflatoxin in peanuts. Even with the use of a small sample size (0.454 kg), the sampling variation was not the largest source of error as found in other mycotoxin test procedures.

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Thomas B Whitaker, Winston M Hagler, Francis G Giesbrecht, Anders S Johansson. 2002. Sampling wheat for deoxynivalenol.. https://doi.org/10.1007/978-1-4615-0629-4_8

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