Rapid compaction during RNA folding.

Rick Russell; Ian S Millett; Mark W Tate; Lisa W Kwok; Bradley Nakatani; Sol M Gruner; Simon G J Mochrie; Vijay Pande; Sebastian Doniach; Daniel Herschlag; Lois Pollack

doi:10.1073/pnas.072589599

PubMed · 11929997

Rapid compaction during RNA folding.

Rick Russell·Ian S Millett·Mark W Tate·Lisa W Kwok·Bradley Nakatani·Sol M Gruner·Simon G J Mochrie·Vijay Pande·Sebastian Doniach·Daniel Herschlag·Lois Pollack

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Abstract

We have used small angle x-ray scattering and computer simulations with a coarse-grained model to provide a time-resolved picture of the global folding process of the Tetrahymena group I RNA over a time window of more than five orders of magnitude. A substantial phase of compaction is observed on the low millisecond timescale, and the overall compaction and global shape changes are largely complete within one second, earlier than any known tertiary contacts are formed. This finding indicates that the RNA forms a nonspecifically collapsed intermediate and then searches for its tertiary contacts within a highly restricted subset of conformational space. The collapsed intermediate early in folding of this RNA is grossly akin to molten globule intermediates in protein folding.

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Animals Computer Simulation Nucleic Acid Conformation RNA, Catalytic Scattering, Radiation Tetrahymena Journal Article

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Rick Russell, Ian S Millett, Mark W Tate, Lisa W Kwok, Bradley Nakatani, Sol M Gruner, Simon G J Mochrie, Vijay Pande, Sebastian Doniach, Daniel Herschlag, Lois Pollack. 2002-04-02. Rapid compaction during RNA folding.. https://doi.org/10.1073/pnas.072589599

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