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Nuclear receptor function requires a TFTC-type histone acetyl transferase complex.

Junn Yanagisawa·Hirochika Kitagawa·Mitsuaki Yanagida·Osamu Wada·Satoko Ogawa·Madoka Nakagomi·Hajime Oishi·Yasuji Yamamoto·Hiromich Nagasawa·Steven B McMahon·Michael D Cole·Laszlo Tora·Nobuhiro Takahashi·Shigeaki Kato
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Abstract

Nuclear receptors (NRs) regulate transcription in a ligand-dependent way through two types of coactivator complexes: the p160/CBP histone acetyl transferase (HAT) complex and the DRIP/TRAP/SMCC complex without HAT activity. Here we identified a large human (h) coactivator complex necessary for the estrogen receptor alpha (ERalpha) transactivation. This complex contains the GCN5 HAT, the c-Myc interacting protein TRRAP/PAF400, TAF(II)30, and other subunits. Similarly to known TFTC (TBP-free TAF(II)-containing)-type HAT complexes (hTFTC, hPCAF, and hSTAGA), TRRP directly interacted with liganded ER alpha, or other NRs. ER alpha transactivation was enhanced by the purified complex in vitro. Antisense TRRAP RNA inhibited estrogen-dependent cell growth of breast cancer cells. Thus, the isolated TFTC-type HAT complex acts as a third class of coactivator complex for NR function.

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AcetyltransferasesAdaptor Proteins, Signal TransducingAmino Acid MotifsCell Cycle ProteinsCell LineCell NucleusEstrogen Receptor alphaHistone AcetyltransferasesHumansLigandsMacromolecular SubstancesNuclear ProteinsProtein BindingRNA, AntisenseReceptors, Cytoplasmic and NuclearReceptors, EstrogenRecombinant Fusion ProteinsSaccharomyces cerevisiae ProteinsTrans-ActivatorsTranscription FactorsTranscriptional Activationp300-CBP Transcription Factorsp300-CBP-Associated FactorJournal ArticleResearch Support, Non-U.S. Gov'tRetracted Publication

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Junn Yanagisawa, Hirochika Kitagawa, Mitsuaki Yanagida, Osamu Wada, Satoko Ogawa, Madoka Nakagomi, Hajime Oishi, Yasuji Yamamoto, Hiromich Nagasawa, Steven B McMahon, Michael D Cole, Laszlo Tora, Nobuhiro Takahashi, Shigeaki Kato. 2002. Nuclear receptor function requires a TFTC-type histone acetyl transferase complex.. https://doi.org/10.1016/s1097-2765(02)00478-1

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