Making ends meet in genetic analysis using padlock probes.

Mats Nilsson; Johan Banér; Maritha Mendel-Hartvig; Fredrik Dahl; Dan-Oscar Antson; Mats Gullberg; Ulf Landegren

doi:10.1002/humu.10073

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Making ends meet in genetic analysis using padlock probes.

Mats Nilsson·Johan Banér·Maritha Mendel-Hartvig·Fredrik Dahl·Dan-Oscar Antson·Mats Gullberg·Ulf Landegren

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Abstract

Padlock probes are molecular tools that combine highly specific target sequence recognition with the potential for multiplexed analysis of large sets of target DNA or RNA sequences. In this brief review, we exemplify the ability of these probes to distinguish single-nucleotide target sequence variants. We further discuss means to detect the location of target sequences in situ, and to amplify reacted padlock probes via rolling-circle replication, as well as to sort reaction products on tag-arrays. We argue that the probes have the potential to render high-throughput genetic analyses precise and affordable.

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DNA DNA Ligases DNA Probes DNA Replication Models, Molecular Nucleic Acid Conformation Nucleic Acid Hybridization Polymerase Chain Reaction RNA Sensitivity and Specificity Substrate Specificity Journal Article Research Support, Non-U.S. Gov't Review

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Mats Nilsson, Johan Banér, Maritha Mendel-Hartvig, Fredrik Dahl, Dan-Oscar Antson, Mats Gullberg, Ulf Landegren. 2002. Making ends meet in genetic analysis using padlock probes.. https://doi.org/10.1002/humu.10073

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