Potentiometric phosphate-sensing system utilizing phosphate-binding protein.

Izumi Kubo

doi:10.1007/s00216-001-1138-1

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Potentiometric phosphate-sensing system utilizing phosphate-binding protein.

Izumi Kubo

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Abstract

A phosphate-detection system has been developed which uses phosphate-binding protein (PBP) from Escherichia coli. PBP was immobilized on a sheet of nitrocellulose membrane by cross-linking and the membrane potential of the immobilized PBP was measured. The response time of the system to phosphate was 5 min. The response was selective to phosphate among other anions. Under optimum conditions 0.1-1.5 mmol L(-1) phosphate can be determined with this system.

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Biosensing Techniques Calibration Collodion Cross-Linking Reagents Escherichia coli Ion-Selective Electrodes Membranes, Artificial Phosphate-Binding Proteins Phosphates Potentiometry Journal Article

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Izumi Kubo. 2001-12-15. Potentiometric phosphate-sensing system utilizing phosphate-binding protein.. https://doi.org/10.1007/s00216-001-1138-1

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