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PubMed · 11944054

Structural studies on membrane proteins using non-linear spin label EPR spectroscopy.

Tibor Páli·Derek Marsh
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Abstract

Non-linear electron spin resonance (EPR) techniques suitable for measuring proximity relationships in membranes are reviewed. These were developed during the past decade in order to measure changes sensitively in the spin-lattice relaxation time (T1) of nitroxyl spin labels covalently attached to membrane lipids or proteins. In combination with paramagnetic quenching agents and double spin-labelling, the methods were further developed for distance measurements. Selected examples are given to illustrate different methods, and types of data obtained for both integral and peripheral membrane proteins.

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Electron Spin Resonance SpectroscopyMembrane LipidsMembrane ProteinsNonlinear DynamicsProtein ConformationSpin LabelsJournal ArticleResearch Support, Non-U.S. Gov't

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Tibor Páli, Derek Marsh. 2002. Structural studies on membrane proteins using non-linear spin label EPR spectroscopy.. https://pubmed.ncbi.nlm.nih.gov/11944054/

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Electron Spin Resonance Spectroscopy↗
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Electron Spin Resonance Spectroscopy↗