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Evidence for a pre-restriction point Cdk3 activity.

Susan M Keezer·David M Gilbert
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Abstract

We have examined the activity of cyclin-dependent kinase 3 (cdk3) during G1-phase of the cell cycle in Chinese Hamster Ovary (CHO) fibroblasts. Histone H1 kinase activity associated with anti-cdk3 immunoprecipitates peaked during a brief window of time, 2-3 h prior to the restriction point. In vitro cdk3 activity was sensitive to roscovitine, a drug previously shown to inhibit cdks 1, 2, and 5, but not cdk4 or 6. Early G1-phase activation of cdk3 was downregulated by treatment of cells with MG132, an inhibitor of the proteasome, and by the protein synthesis inhibitor cycloheximide. These results provide evidence for a pre-restriction point cdk3 activity that requires both the synthesis of a regulatory subunit and degradation of an inhibitor.

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AnimalsCHO CellsCells, CulturedCricetinaeCyclin-Dependent KinasesCycloheximideCysteine EndopeptidasesEnzyme InhibitorsG1 PhaseLeupeptinsMultienzyme ComplexesPrecipitin TestsProteasome Endopeptidase ComplexProtein KinasesProtein Synthesis InhibitorsPurinesReplication OriginRoscovitineJournal ArticleResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, P.H.S.

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Susan M Keezer, David M Gilbert. 2002. Evidence for a pre-restriction point Cdk3 activity.. https://doi.org/10.1002/jcb.10162

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