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Direct fluorescence polarization immunoassay.

Y Kobayashi·K Miyai·N Tsubota·F Watanabe
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Abstract

Direct fluorescence polarization immunoassay of serum cortisol was established. Non-specific binding of fluorescent-labelled cortisol to serum proteins was successively eliminated by sodium dodecyl sulfate. The minimal amount of cortisol detected was 0.1 ng/tube and serum concentration of 1.0 microgram/dl to 100 microgram/dl of cortisol could be measured. This fluorescence polarization immunoassay satisfied the standard criteria of accuracy and precision. The values correlated well with those obtained by radioimmunoassay.

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Fluorescent Antibody TechniqueHumansHydrocortisonePepsin ARadioimmunoassayComparative StudyJournal Article

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BibTeXRIS

Y Kobayashi, K Miyai, N Tsubota, F Watanabe. 1979. Direct fluorescence polarization immunoassay.. https://doi.org/10.1016/0039-128x(79)90095-3

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