A simple method for the quantitation of isozyme patterns.

R J Klebe

doi:10.1007/bf00484412

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A simple method for the quantitation of isozyme patterns.

R J Klebe

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Abstract

Isozyme patterns may be analyzed quantitatively, without the use of a densitometer, by performing serial twofold dilutions of a sample to a visual end point. The specific activity (S) of a given dehydrogenase isozyme can be assessed in the presence of other isozymes catalyzing the same reaction, by (1) determining the isozyme titer (T) (defined as mg protein/ml in the last visible band) and (2) applying the formula S = K/T, where K is 1.6 X 10(-3) units/ml in the last visible band. The units/ml (U) in the starting material can be calculated from the equation U = K (2)n-1, where n is the number of the slot producing the last visible band.

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Animals Blood Proteins Electrophoresis, Starch Gel Erythrocytes Genetic Variation Glucose-6-Phosphate Isomerase Isoenzymes L-Lactate Dehydrogenase Mice Mice, Inbred BALB C Mice, Inbred C3H Mice, Inbred Strains Organ Specificity Species Specificity Journal Article

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R J Klebe. 1975. A simple method for the quantitation of isozyme patterns.. https://doi.org/10.1007/bf00484412

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