Integration host factor interactions with Neisseria gene sequences: correlation between predicted binding sites and in vitro binding of Neisseria -derived IHF protein.

S A Hill; D S Samuels; C Nielsen; S W Knight; F Pagotto; J A R Dillon

doi:10.1006/mcpr.2001.0403

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Integration host factor interactions with Neisseria gene sequences: correlation between predicted binding sites and in vitro binding of Neisseria -derived IHF protein.

S A Hill·D S Samuels·C Nielsen·S W Knight·F Pagotto·J A R Dillon

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Abstract

Putative integration host factor (IHF) binding sites are frequently being identified in Neisseria gene sequences on the basis of similarity to a degenerate Escherichia coli -derived consensus binding sequence. In this report, three different Neisseria genetic systems that contain predicted IHF binding sites were assessed for IHF binding through gel retardation analysis. The results show a positive correlation between the identification of a predicted Neisseria IHF binding site and in vitro binding of Neisseria -derived IHF protein.

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Base Sequence Binding Sites DNA, Bacterial Integration Host Factors Molecular Sequence Data Neisseria Promoter Regions, Genetic Protein Binding Sequence Alignment Statistics as Topic Journal Article Research Support, U.S. Gov't, Non-P.H.S.

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S A Hill, D S Samuels, C Nielsen, S W Knight, F Pagotto, J A R Dillon. 2002. Integration host factor interactions with Neisseria gene sequences: correlation between predicted binding sites and in vitro binding of Neisseria -derived IHF protein.. https://doi.org/10.1006/mcpr.2001.0403

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