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Stability of gene expression in postmortem brain revealed by cDNA gene array analysis.

Stacey A Trotter·Louis B Brill·James P Bennett
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Abstract

We utilized nylon arrays to measure gene expression in mouse brains after various postmortem intervals (PMIs). Gene expression after overnight refrigeration or 4 h at room temperature then overnight refrigeration correlated highly and approximately equivalently to that in brains processed immediately. After 8-24 h at room temperature and overnight refrigeration gene expression correlation and equivalency declined, but 90-95% of detected genes were within +/-40% of baseline levels. Brain homogenate pH did not change with PMI.

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AnimalsBrain ChemistryGene ExpressionGene Expression ProfilingMiceOligonucleotide Array Sequence AnalysisPostmortem ChangesRNA StabilityRNA, MessengerJournal ArticleResearch Support, U.S. Gov't, P.H.S.

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Stacey A Trotter, Louis B Brill, James P Bennett. 2002-06-28. Stability of gene expression in postmortem brain revealed by cDNA gene array analysis.. https://doi.org/10.1016/s0006-8993(02)02644-6

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