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Rat interleukin-18 binding protein: cloning, expression, and characterization.

Sin-Hyeog Im·Soo-Hyun Kim·Tania Azam·Natarajan Venkatesh·Charles A Dinarello·Sara Fuchs·Miriam C Souroujon
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Abstract

Interleukin-18 binding protein (IL-18BP) is a constitutively expressed and secreted protein that lacks a transmembrane domain. IL-18BP binds specifically to mature IL-18 and inhibits its activity. To study the immunomodulating role of IL-18BP in models of autoimmune diseases in rats, we cloned and characterized rat IL-18BP. Rat IL-18BP has 193 amino acid residues and is highly homologous to human and mouse IL-18BP. Recombinant rat IL-18BP binds to rat IL-18, reacts with antibodies to human or mouse IL-18BP, and inhibits IL-18-dependent interferon-gamma (IFN-gamma) production in vitro. Thus, rat IL-18BP can be employed to antagonize the proinflammatory responses induced by endogenous IL-18 in rat models of autoimmune diseases.

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Amino Acid SequenceAnimalsBase SequenceCOS CellsCloning, MolecularDose-Response Relationship, DrugEscherichia coliGene ExpressionGlycoproteinsHumansIntercellular Signaling Peptides and ProteinsInterferon-gammaInterleukin-12Interleukin-18Molecular Sequence DataRatsRats, Inbred LewRecombinant ProteinsSequence Homology, Amino AcidJournal ArticleResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, P.H.S.

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Sin-Hyeog Im, Soo-Hyun Kim, Tania Azam, Natarajan Venkatesh, Charles A Dinarello, Sara Fuchs, Miriam C Souroujon. 2002. Rat interleukin-18 binding protein: cloning, expression, and characterization.. https://doi.org/10.1089/107999002753675749

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