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PubMed · 12034817

Tiny telomere DNA.

Jinsong Ren·Xiaogang Qu·John O Trent·Jonathan B Chaires
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Abstract

We describe the design, synthesis and biophysical characterization of a novel DNA construct in which a folded quadruplex structure is joined to a standard double helix. Circular dichroism, gel electrophoresis, three-dimensional UV melting and differential scanning calorimetry were all used to characterize the structure. Rigorous molecular dynamics simulations were used to build a plausible atomic-level structural model of the DNA construct. This novel DNA construct provides a model for the duplex-quadruplex junction region at the end of chromosomal DNA and offers a system for the study of structure-selective ligand binding.

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Base SequenceCalorimetry, Differential ScanningCircular DichroismDNAG-QuadruplexesLigandsModels, MolecularNucleic Acid ConformationNucleic Acid DenaturationOligodeoxyribonucleotidesSpectrometry, FluorescenceTelomereThermodynamicsUltraviolet RaysJournal ArticleResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, P.H.S.

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Jinsong Ren, Xiaogang Qu, John O Trent, Jonathan B Chaires. 2002-06-01. Tiny telomere DNA.. https://doi.org/10.1093/nar%2F30.11.2307

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