Protease-containing silicates as active antifouling materials.

Jungbae Kim; Ray Delio; Jonathan S Dordick

doi:10.1021/bp020036q

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Protease-containing silicates as active antifouling materials.

Jungbae Kim·Ray Delio·Jonathan S Dordick

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Abstract

Biocatalytic silicates, composite materials composed of alpha-chymotrypsin and a silicate prepolymer, were prepared via a two-step polymerization process following solubilization of the enzyme in the polymerization media. This new approach resulted in active and stable composites, and a calculated half-life of over 350 days in aqueous buffer at 30 degrees C. The high stability and activity of this biocatalytic silicate was likely due to the covalent attachment between alpha-chymotrypsin and the silicate matrix. The protease-containing silicate was resistant to fouling by nonselective protein binding, as demonstrated by the dramatically reduced binding of human serum albumin to the silicate material when compared to that of a silicate containing pre-inactivated alpha-chymotrypsin.

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Catalysis Chymotrypsin Silicates Journal Article Research Support, U.S. Gov't, Non-P.H.S.

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Jungbae Kim, Ray Delio, Jonathan S Dordick. Protease-containing silicates as active antifouling materials.. https://doi.org/10.1021/bp020036q

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