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Analyzing mitochondrial changes during apoptosis.

Roberta A Gottlieb·David J Granville
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Abstract

Mitochondria play a central role in programmed cell death through the release of cytochrome c and other proapoptotic factors. Fluorescence microscopy is used to visualize cytochrome c translocation and loss of mitochondrial membrane potential. Flow cytometry can also be used to measure mitochondrial membrane potential. Cytochrome c content in cytosol and mitochondria can be determined by immunoblotting after subcellular fractionation or selective permeabilization with digitonin. Isolated mitochondria can be used to study the mechanism of cytochrome c release. This article summarizes some of the more widely used methods to assess mitochondrial alterations in apoptosis.

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ApoptosisBH3 Interacting Domain Death Agonist ProteinCarrier ProteinsCell DeathCell-Free SystemCytochrome c GroupCytosolDose-Response Relationship, DrugFlow CytometryGreen Fluorescent ProteinsHeLa CellsHumansImmunoblottingJurkat CellsLuminescent ProteinsMembrane PotentialsMicroscopy, FluorescenceMitochondriaPotassiumProtein TransportProto-Oncogene ProteinsProto-Oncogene Proteins c-bcl-2Recombinant Fusion ProteinsSubcellular Fractionsbcl-2-Associated X ProteinJournal Article

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Roberta A Gottlieb, David J Granville. 2002. Analyzing mitochondrial changes during apoptosis.. https://doi.org/10.1016/s1046-2023(02)00040-3

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Apoptosis↗