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PubMed · 12059207

Phospholipid/protein cones.

Bijaya K Mishra·Britt N Thomas
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Abstract

The presence of protein in tubule-forming solutions of the diacetylenic phospholipid 1,2-bis(10,12-tricosadiynoyl)-sn-glycero-3-phosphocholine results in the formation of hollow cones rather than the expected hollow cylinders. Differential phase-contrast video microscopy reveals that cones grow from proteinaceous nodules in a fashion similar to cylindrical tubule growth from spherical vesicles. Spatially resolved electron-beam energy-dispersive X-ray fluorescence spectroscopy shows the protein to be associated with the cone wall. Small-angle X-ray scattering shows that, like the protein-free cylinders, the cones are multilamellar with essentially identical interlamellar spacing.

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DiynesMicroscopy, Atomic ForceMicroscopy, Electron, ScanningMuramidasePhosphatidylcholinesPhospholipidsPhosphorylcholineScattering, RadiationX-RaysJournal ArticleResearch Support, U.S. Gov't, Non-P.H.S.

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Bijaya K Mishra, Britt N Thomas. 2002-06-19. Phospholipid/protein cones.. https://doi.org/10.1021/ja020067h

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