Towards quantitative assays with peptide chips: a surface engineering approach.

Benjamin T Houseman; Milan Mrksich

doi:10.1016/s0167-7799(02)01984-4

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Towards quantitative assays with peptide chips: a surface engineering approach.

Benjamin T Houseman·Milan Mrksich

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Abstract

The development of peptide and protein microarrays has created enormous opportunities in biomedical research. Current chip-based assays are well suited for identifying candidate protein or enzyme activities but still require conventional solution phase experiments to validate hits. Here, three surface-engineering strategies for microarray design are described and are illustrated in the development of a peptide chip for the quantitative analysis of kinase activity on solid support. These strategies promise to widen the application of microarrays by permitting the evaluation of hits in a chip-based format.

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Amino Acid Sequence Enzymes, Immobilized Molecular Sequence Data Peptides Protein Array Analysis Protein-Tyrosine Kinases Substrate Specificity Surface Properties Journal Article

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Benjamin T Houseman, Milan Mrksich. 2002. Towards quantitative assays with peptide chips: a surface engineering approach.. https://doi.org/10.1016/s0167-7799(02)01984-4

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