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PubMed · 12064657

Typing Y chromosome STR haplotypes using redesigned primers.

Yiping Hou·Ji Zhang·Dahong Sun·Yinbi Li·Jin Wu·Sizhong Zhang·Mechthild Prinz
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Abstract

A panel of Y-specific STR loci, including DYS19, DYS389, DYS390, DYS391, DYS392, and DYS393 was analyzed using horizontal nondenaturing polyacrylamide gel electrophoresis with a discontinuous buffer system (horizontal disk-PAGE). In order to obtain correct results for the larger DYS389 and DYS392 alleles, it was necessary to design new primers that bind closer to the repeat region and lead to a significant reduction of the amplified fragment size. Using the modified primer sets the horizontal disk-PAGE results were consistent with a nondenaturing approach using fluorescent primers and a 377 automated sequencer. The modified procedure also amplifies the second repeat stretch at the duplicated DYS389 locus as a single fragment, which results in an immediate allele identification. The results indicate that horizontal disk-PAGE with silverstaining is a simple approach to type the recommended Y-specific STR markers.

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DNA PrimersElectrophoresis, Polyacrylamide GelForensic MedicineHaplotypesHumansMaleTandem Repeat SequencesY ChromosomeJournal ArticleResearch Support, Non-U.S. Gov't

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Yiping Hou, Ji Zhang, Dahong Sun, Yinbi Li, Jin Wu, Sizhong Zhang, Mechthild Prinz. 2002. Typing Y chromosome STR haplotypes using redesigned primers.. https://pubmed.ncbi.nlm.nih.gov/12064657/

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