SKIP is an indispensable factor for Caenorhabditis elegans development.

Marta Kostrouchova; Daniel Housa; Zdenek Kostrouch; Vladimir Saudek; Joseph Edward Rall

doi:10.1073/pnas.112213799

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SKIP is an indispensable factor for Caenorhabditis elegans development.

Marta Kostrouchova·Daniel Housa·Zdenek Kostrouch·Vladimir Saudek·Joseph Edward Rall

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Abstract

SKI-binding protein (SKIP) is a transcription cofactor present in all eukaryotes. Here we show that SKIP is a unique protein that is required for Caenorhabditis elegans viability and development. Expression of CeSKIP (skp-1) assayed by RT-PCR and by GFP fluorescence in transgenic lines starts in embryos and continues to adulthood. Loss of CeSKIP activity by RNA-mediated inhibition results in early embryonic arrest similar to that seen following inhibition of RNA polymerase II. RNA polymerase II phosphorylation appears normal early in CeSKIP RNA-mediated inhibition treated embryos although the expression of several embryonic GFP reporter genes is severely restricted or absent. Our data suggest that CeSKIP is an essential component of many RNA polymerase II transcription complexes and is indispensable for C. elegans development.

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Animals Animals, Genetically Modified Caenorhabditis elegans Caenorhabditis elegans Proteins Gene Expression Regulation, Developmental Genes, Helminth Helminth Proteins Larva Nuclear Proteins RNA Polymerase II RNA, Fungal Transcription Factors Ubiquitin-Protein Ligase Complexes Journal Article Research Support, Non-U.S. Gov't

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Marta Kostrouchova, Daniel Housa, Zdenek Kostrouch, Vladimir Saudek, Joseph Edward Rall. 2002-06-25. SKIP is an indispensable factor for Caenorhabditis elegans development.. https://doi.org/10.1073/pnas.112213799

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