[Interactions between liposomes and synaptosomes].

L Foia; M Costuleanu; N Costuleanu; M Pavel; D Ungureanu; F Filip

PubMed · 12092138

[Interactions between liposomes and synaptosomes].

L Foia·M Costuleanu·N Costuleanu·M Pavel·D Ungureanu·F Filip

Abstract

In continuing experiments to determine the basis of presynaptic modulation, rat cortical synaptosomes were employed and transmembrane calcium fluxes were determined with a metalocromophoric dye, Arsenazo III. The intracellular free Ca2+ concentrations show complex fluctuations in time and space in response to a variety of stimuli, acting as a pluripotent signal for many neuronal functions. Depolarisation of synaptosomes by K+ 20 mM determined calcium influx in cortical synaptosomes, mainly mediated by the Q type Ca2+ channels, with a minor participation of the N type channels. The administration protocol for the polyamines, revealed that agmatine, spermine, spermidine, putresceine and cadaverine reduce, in variable proportions, the K(+)-induced Ca2+ influx, in synaptosomal preparations. The results plead for the ability of agmatine and spermine to modulate Ca2+ fluxes in synaptosomes, both from the interior and exterior side. Moreover, rigidization of the synaptosomal membrane (by cholesterol-enriched liposomes administration) do not significantly influences K(+)-induced Ca2+ influx in synaptosomal preparations.

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Animals Arsenazo III Calcium Channels Cerebral Cortex Coloring Agents Liposomes Male Polyamines Presynaptic Terminals Rats Spermine Synaptosomes English Abstract Journal Article

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L Foia, M Costuleanu, N Costuleanu, M Pavel, D Ungureanu, F Filip. [Interactions between liposomes and synaptosomes].. https://pubmed.ncbi.nlm.nih.gov/12092138/

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