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Cutting edge: CCR7+ and CCR7- memory T cells do not differ in immediate effector cell function.

Heike Unsoeld·Stefan Krautwald·David Voehringer·Ulrich Kunzendorf·Hanspeter Pircher
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Abstract

It has been proposed that expression of the chemokine receptor CCR7 represents a defining factor for nonpolarized central (CCR7(+)) and polarized effector memory (CCR7(-)) T cells. In this study, we have tested this hypothesis using in vivo-activated T cells from P14 and SMARTA TCR-transgenic (tg) mice specific for MHC class I- and II-restricted epitopes of the lymphocytic choriomeningitis virus (LCMV) glycoprotein. CCR7 cell surface expression on TCR-tg cells was monitored with a CC chemokine ligand 19-Ig fusion protein. CC chemokine ligand 19-Ig staining separated TCR-tg cells activated by LCMV infection into CCR7(-) and CCR7(+) effector/memory T cell populations. Nonetheless, both T cell populations isolated from spleen and liver produced identical amounts of IFN-gamma after short-term Ag stimulation. Furthermore, CCR7(+) and CCR7(-) CD8 TCR-tg cells from LCMV-infected mice exhibited similar lytic activity against LCMV peptide-coated target cells. These results question the proposed concept of differential effector cell function of CCR7(+) and CCR7(-) memory T cells.

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Amino Acid SequenceAnimalsAntigens, ViralCD4-Positive T-LymphocytesCD8-Positive T-LymphocytesCell MembraneCytokinesCytotoxicity, ImmunologicEpitopes, T-LymphocyteGlycoproteinsImmunologic MemoryKineticsLymphocyte ActivationLymphocytic choriomeningitis virusMiceMice, Inbred C57BLMice, KnockoutMice, TransgenicMolecular Sequence DataPeptide FragmentsReceptors, CCR7Receptors, ChemokineT-Lymphocyte SubsetsViral ProteinsComparative StudyJournal ArticleResearch Support, Non-U.S. Gov't

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Heike Unsoeld, Stefan Krautwald, David Voehringer, Ulrich Kunzendorf, Hanspeter Pircher. 2002-07-15. Cutting edge: CCR7+ and CCR7- memory T cells do not differ in immediate effector cell function.. https://doi.org/10.4049/jimmunol.169.2.638

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