Probing functionalized gold colloids for single particle tracking experiments.

Frédéric Daumas; Honoré Mazarguil; Claire Millot; André Lopez; Laurence Salomé

doi:10.1016/s0006-291x(02)00722-2

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Probing functionalized gold colloids for single particle tracking experiments.

Frédéric Daumas·Honoré Mazarguil·Claire Millot·André Lopez·Laurence Salomé

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Abstract

Functionalized submicroscopic particles are currently used to label proteins or lipids at the surface of living cells for single particle tracking experiments. In many cases, it can be of crucial importance for the particle to be anchored to a single molecule. We have addressed this question for the labeling at the plasma membrane of NRK cells of the mu-opioid receptor bearing a T7 epitope at the N-terminus. Using biophysical methods we were able to prepare quasi-monovalent anti-T7 antibody conjugated gold colloids (40 nm diameter) leading to stable and specific binding to the receptor. The rational method, we report here, can be extended to design customized probes for the labeling of various tagged molecules.

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Animals Binding Sites Cell Line Cell Membrane Colloids Gold Hydrogen-Ion Concentration Immunoglobulin Fragments Isoelectric Point Kinetics Lipids Microscopy Rats Receptors, Opioid, mu Sodium Surface Plasmon Resonance Time Factors Transfection Journal Article

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Frédéric Daumas, Honoré Mazarguil, Claire Millot, André Lopez, Laurence Salomé. 2002-07-19. Probing functionalized gold colloids for single particle tracking experiments.. https://doi.org/10.1016/s0006-291x(02)00722-2

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