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Capillary electrophoretic detection in apolipoprotein E genotyping.

Otto Bekers·Robert A M op den Buijsch·Johan E de Vries·Petal A H M Wijnen·Marja P van Dieijen-Visser
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Abstract

We describe the application of capillary electrophoresis to detect DNA fragments, obtained after amplifying a part of the apolipoprotein E (apoE) gene with polymerase chain reaction (PCR). Compared to conventional agarose slab gel electrophoresis (AGE), CE appears the method of choice with regard to resolution and sensitivity, to detect DNA fragments in the range of 20-100 base pairs. Especially discrimination between apoE2/E2 and apoE2/E3 genotypes is more reliable with CE than with AGE, this being of great clinical value in the diagnosis of familiary dysbetalipoproteinemia.

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Apolipoprotein E2Apolipoprotein E3Apolipoproteins EDNAElectrophoresis, Agar GelElectrophoresis, CapillaryGenotypePolymerase Chain ReactionSensitivity and SpecificityTime FactorsJournal Article

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BibTeXRIS

Otto Bekers, Robert A M op den Buijsch, Johan E de Vries, Petal A H M Wijnen, Marja P van Dieijen-Visser. 2002. Capillary electrophoretic detection in apolipoprotein E genotyping.. https://doi.org/10.1002/1522-2683(200206)23%3A12%3C1878%3A%3Aaid-elps1878%3E3.0.co%3B2-g

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