PubMed · 1211626
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K C Chen, R M Krause. 1975. A peptide mapping technique--a three map system.. https://doi.org/10.1016/0003-2697(75)90579-5
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A version of isotope dilution mass spectrometric technique elaborated for measuring endogenous lithium concentrations in human blood plasma and urine (10(-7) M region) and applicable in clinical practice is described. A tracer solution of lithium (LiCl) enriched in 6Li (with abundances 6Li 92%, 7Li 8%) is added to a certain volume of human plasma (0.2-0.4 ml) or urine (0.05-0.1 ml) and dried under an infrared lamp. Thereupon a soluble part extracted from dried plasma with the aid of 0.1 N HCl is dried as well. Dry extracts from plasma and dried samples of urine are processed by 30% H2O2 and subsequently subjected to paper chromatography (with ethanol as a solvent). Such processing of samples is simple and short (about an hour, 3 min of chromatographic process inclusive), achieving a good separation from organic matrix and interfering macroelements. Contamination of sample in processing is about 2-5 pmol; routine control of contamination and account of their influence are accomplished by measuring two different volumes of each sample. Measurements are made with high precision: cyclic repeated scanning of 7Li and 6Li peaks have a standard deviation of 7Li/6Li ratio no more than 0.7%. The method described was used to determine endogenous lithium clearance of hypertensive patients and patients with transplanted kidney.
TLC and PC have been used in the qualitative study of huaijiao wan. The contents of the total flavones and volatile oils in huaijiao wan have been determined. The proposed method can be used to control the quality of huaijiao wan.