A nucleic acid sequence-based amplification method to detect Salmonella enterica serotype enteritidis strain PT4 in liquid whole egg.

N Cook; J Ellison; A S Kurdziel; S Simpkins; J P Hays

doi:10.4315/0362-028x-65.7.1177

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A nucleic acid sequence-based amplification method to detect Salmonella enterica serotype enteritidis strain PT4 in liquid whole egg.

N Cook·J Ellison·A S Kurdziel·S Simpkins·J P Hays

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Abstract

Nucleic acid sequence-based amplification (NASBA) was applied to the detection of Salmonella enterica cells in liquid whole egg. Samples (25 g) of liquid whole egg inoculated with Salmonella cells were enriched for 16 h in buffered peptone water, and the NASBA procedure was effective in detecting the presence of Salmonella in samples inoculated with 10 to 100 CFU prior to enrichment.

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Animals Colony Count, Microbial Eggs Food Microbiology Nucleic Acid Amplification Techniques RNA, Bacterial Salmonella enteritidis Self-Sustained Sequence Replication Sequence Analysis, RNA Journal Article Research Support, Non-U.S. Gov't

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N Cook, J Ellison, A S Kurdziel, S Simpkins, J P Hays. 2002. A nucleic acid sequence-based amplification method to detect Salmonella enterica serotype enteritidis strain PT4 in liquid whole egg.. https://doi.org/10.4315/0362-028x-65.7.1177

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