Multiple Rabaptin-5-like transcripts.

Elena V Korobko; Sergey L Kiselev; Igor V Korobko

doi:10.1016/s0378-1119(02)00677-7

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Multiple Rabaptin-5-like transcripts.

Elena V Korobko·Sergey L Kiselev·Igor V Korobko

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Abstract

Rabaptin-5 is an important component of intracellular vesicular transport machinery. In this report, we describe a novel Rabaptin-5gamma transcript which has a 120 nt deletion in coding region of Rabaptin-5 messenger RNA (mRNA). We also cloned the 5'part of Rabaptin-5delta transcript and showed that upstream previously described deletion, Rabaptin-5delta mRNA is identical to Rabaptin-5. Both deletions are in-frame and result in excision of 40 and 43 aa, respectively, from Rabaptin-5 polypeptide chain. We also detected Rabaptin-5delta and Rabaptin-5gamma transcripts in human. The results of Southern blot analysis and human genome sequence analysis strongly suggest that these transcripts along with Rabaptin-5 and Rabaptin-4 are encoded by a single gene, RAB5EP, and differ due to alternative pre-mRNA splicing. Although Rabaptin-5delta and Rabaptin-5gamma transcripts are low-abundant compared to Rabaptin-5 mRNA, their ubiquitous expression patterns might point on significant roles played by encoded by them proteins.

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Alternative Splicing Animals Base Sequence Carrier Proteins Exons Gene Expression Genes Humans Introns Membrane Proteins Mice Molecular Sequence Data Protein Isoforms RNA, Messenger Transcription, Genetic Vesicular Transport Proteins Journal Article Research Support, Non-U.S. Gov't

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Elena V Korobko, Sergey L Kiselev, Igor V Korobko. 2002-06-12. Multiple Rabaptin-5-like transcripts.. https://doi.org/10.1016/s0378-1119(02)00677-7

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