Streptococcus mutans binding to solid phase dextran mediated by the glucan-binding protein C.

Y Sato; H Senpuku; K Okamoto; N Hanada; H Kizaki

doi:10.1034/j.1399-302x.2002.170408.x

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Streptococcus mutans binding to solid phase dextran mediated by the glucan-binding protein C.

Y Sato·H Senpuku·K Okamoto·N Hanada·H Kizaki

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Abstract

Streptococcus mutans GbpC is a wall-anchored surface protein which is involved in dextran-dependent aggregation. The GbpC phenotype is observed only in cells grown under stress conditions. In order to detect the GbpC protein of S. mutans, we isolated the wall fraction following digestion of the cell wall of this organism by N-acetylmuramidase, and detected the GbpC protein from S. mutans cells by western analysis with anti-GbpC serum. Interestingly, S. mutans cells exhibiting the negative dextran(alpha-1,6 glucan)-dependent aggregation (ddag) phenotype expressed the protein and could bind to immobilized dextran.

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Bacterial Adhesion Bacterial Proteins Blotting, Western Carrier Proteins Cell Wall Dextrans Electrophoresis, Polyacrylamide Gel Lectins Streptococcus mutans Journal Article Research Support, Non-U.S. Gov't

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Y Sato, H Senpuku, K Okamoto, N Hanada, H Kizaki. 2002. Streptococcus mutans binding to solid phase dextran mediated by the glucan-binding protein C.. https://doi.org/10.1034/j.1399-302x.2002.170408.x

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