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Ribosomal highly basic 23-kDa protein as a reliable standard for gene expression analysis.

R Jesnowski·C Backhaus·J Ringel·M Löhr
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Abstract

BACKGROUND/AIMS: Analysis of gene expression is dependent on normalization using housekeeping genes. However, many of these housekeeping genes (e.g. GAPDH, beta-actin) are upregulated in chronic pancreatitis and pancreatic cancer, and cannot be used for normalization. For this reason we tried to identify a housekeeping gene useful for expression analysis in pancreatic diseases. METHODS: RNA isolated from various tissues and states of disease was subjected to reverse transcription and subsequently amplified by PCR using primers for GAPDH and for the ribosomal highly basic 23-kDa (rb 23-kDa, RPL13A) protein. RESULTS: As anticipated, expression of GAPDH varied markedly in the different tissues, whereas the expression of rb 23-kDa was constant in all samples investigated. CONCLUSION: We recommend the use of the ribosomal highly basic 23-kDa protein as a standard for normalization at least for the pancreas and the prostate.

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Gene ExpressionGlyceraldehyde-3-Phosphate DehydrogenasesHumansMolecular WeightPancreatic DiseasesRNA, MessengerReference StandardsReverse Transcriptase Polymerase Chain ReactionRibosomal ProteinsJournal ArticleResearch Support, Non-U.S. Gov't

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R Jesnowski, C Backhaus, J Ringel, M Löhr. 2002. Ribosomal highly basic 23-kDa protein as a reliable standard for gene expression analysis.. https://doi.org/10.1159/000065091

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