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PubMed · 12151767

BDNF binding to truncated trkB.T1 does not affect gene expression.

Nina Offenhäuser·Valeria Muzio·Stefano Biffo
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Abstract

Truncated trkB.T1 is a splice variant of the neurotrophin receptor trkB. In spite of its abundance, and ability to bind and internalize BDNF, it is not clear whether it can transmit BDNF signaling. We tested this hypothesis by searching for proteins binding the evolutionarily conserved cyto-domain of trkB.T1, and by studying BDNF-induced changes of gene expression through DNA microarrays. Cells bearing trkB.T1 receptors presented morphological changes. However, no cytoplasmic interactors of trkB.T1 were found. In addition, BDNF-dependent modulation of gene expression was detected in cells bearing trkB.TK but not trkB.T1 receptors. These results suggest that the main function of trkB.T1 is to regulate local availability of neurotrophins and that it is unable to sense changes in BDNF availability.

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3T3 CellsAlternative SplicingAnimalsBrain-Derived Neurotrophic FactorCell SizeEndocytosisGene ExpressionGenesMiceNervous SystemNervous System DiseasesNeuronal PlasticityNeuronsNeuroprotective AgentsProtein BindingProtein IsoformsProtein Structure, TertiaryRNA, MessengerReceptor, trkBReceptors, Cell SurfaceSignal TransductionTranscription, GeneticTransduction, GeneticJournal ArticleResearch Support, Non-U.S. Gov't

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Nina Offenhäuser, Valeria Muzio, Stefano Biffo. 2002-07-02. BDNF binding to truncated trkB.T1 does not affect gene expression.. https://doi.org/10.1097/00001756-200207020-00024

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