To fabricate artificial nerves with tissue engineering methods.

Biao Cheng; Zhengrong Chen

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PubMed · 12162898

To fabricate artificial nerves with tissue engineering methods.

Biao Cheng·Zhengrong Chen

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Abstract

OBJECTIVE: To fabricate artificial nerves with tissue engineering methods in vitro. METHODS: Schwann cells (SCs) were cultured and seeded on polyglactin 910 fibers wrapped by biomembrane coated with rat tail glue and laminin for 2 weeks. The absorbability on the scaffolds, growth and migration of SCs were assessed with a light microscope, a scanning electron microscope and a transmission electron microscope. RESULTS: SCs could migrate and proliferate on polyglactin 910 fibers. They were well distributed between scaffolds and absorbed on surface of scaffolds and formed a bungner band, on which SCs produced more matrices. SCs seeded on the biomembrane could also grow well. Axon regeneration in the distal nerve stump was observed at 8 weeks. CONCLUSIONS: Adult SCs can be expanded on coated fibers and biomembrane. Three-dimensional scaffold of SCs has the basic characteristics of artificial nerves. These findings offer a novel method to fabricate artificial nerves with tissue engineering methods for repairing defected long nerves.

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Animals Cells, Cultured Male Microscopy, Electron Nerve Regeneration Rabbits Schwann Cells Sciatic Nerve Tissue Engineering Journal Article Research Support, Non-U.S. Gov't

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Biao Cheng, Zhengrong Chen. 2002. To fabricate artificial nerves with tissue engineering methods.. https://pubmed.ncbi.nlm.nih.gov/12162898/

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