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Differential Pax6 promoter activity and transcript expression during forebrain development.

Tonya R Anderson·Eva Hedlund·Ellen M Carpenter
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Abstract

Three different Pax6 promoters -- P0, P1, and P alpha -- show differential activity in the developing eye and spinal cord. To examine promoter usage during forebrain development, we performed in situ hybridization and reverse transcription-polymerase chain reaction to detect transcripts initiated from each promoter. Promoter-specific transcripts are expressed within subdomains of total Pax6 expression, but differ from one another in their spatial localization and expression over time. Additionally, we identified a novel P0-initiated transcript and detected a developmentally regulated antisense transcript.

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Alternative SplicingAnimalsBase SequenceEye ProteinsGene Expression Regulation, DevelopmentalHomeodomain ProteinsIn Situ HybridizationMiceModels, GeneticMolecular Sequence DataPAX6 Transcription FactorPaired Box Transcription FactorsPromoter Regions, GeneticProsencephalonRepressor ProteinsReverse Transcriptase Polymerase Chain ReactionTime FactorsJournal ArticleResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, P.H.S.

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Tonya R Anderson, Eva Hedlund, Ellen M Carpenter. 2002. Differential Pax6 promoter activity and transcript expression during forebrain development.. https://doi.org/10.1016/s0925-4773(02)00051-5

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