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Robust and sensitive nylon hybridization membrane suitable for high-throughput robotic arraying applications.

M T Green·N C Maller·S A Richmond·D Morris·P R Ball·S K Stephens
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Abstract

An important aspect of automated macroarraying is the suitability of the nylon membrane selected on which samples are to be arrayed. PerForma is a positively charged nylon membrane that has been developed specificallyfor automated macroarraying. Tests usingfluorescent hybridization detection methods have shown that immobilized DNA amounts as low as 0.25 pg can be detected and that positive signals are obtainable after 21 stripping cycles. This report describes the improved colony growth, improved handling characteristics, increased hybridization detection sensitivity, and increased stripping and reprobing capability obtained using PerForma.

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Cells, CulturedEscherichia coliGene LibraryLuminescent MeasurementsMembranes, ArtificialNucleic Acid HybridizationOligonucleotide Array Sequence AnalysisQuality ControlReproducibility of ResultsRoboticsSensitivity and SpecificityTransformation, BacterialEvaluation StudyJournal Article

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M T Green, N C Maller, S A Richmond, D Morris, P R Ball, S K Stephens. 2002. Robust and sensitive nylon hybridization membrane suitable for high-throughput robotic arraying applications.. https://doi.org/10.2144/02332ht04

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