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Multistep small-molecule synthesis programmed by DNA templates.

Zev J Gartner·Matthew W Kanan·David R Liu
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Abstract

The translation of DNA sequences into synthetic products is a key requirement of our approach to evolving synthetic molecules through iterated cycles of translation, selection, and amplification. Here we report general linker and purification strategies for sequence-specific DNA-templated synthesis that collectively enable the product of a DNA-templated reaction to be isolated and to undergo subsequent DNA-templated reactions. Using these strategies, we have achieved the first multistep nucleic acid-templated small-molecule syntheses to generate two different molecules. In addition to representing a method for translating DNA templates sequence-specifically into corresponding multistep synthetic products, our findings also provide experimental support for previously proposed models invoking multistep nucleic acid-templated synthesis as mediating the prebiotic translation of replicable information into the earliest functional molecules.

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AmidesDNAOligonucleotidesPeptidesTemplates, GeneticJournal ArticleResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, Non-P.H.S.

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Zev J Gartner, Matthew W Kanan, David R Liu. 2002-09-04. Multistep small-molecule synthesis programmed by DNA templates.. https://doi.org/10.1021/ja027307d

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