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Investigating specific antigen/antibody binding with the atomic force microscope.

O Ouerghi·A Touhami·A Othmane·H Ben Ouada·C Martelet·C Fretigny·N Jaffrezic-Renault
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Abstract

The aim of this work is to detect immune complexes without any kind of labelling of each of the immunological species, with a view to create a very sensitive biosensor. This is achieved by using the atomic force microscopy. We have proceeded by imaging the antibody (anti-rabbit IgG) or anti-rabbit IgG moieties adsorbed onto mica surface, before and after incubation of two kinds of antigens: a specific (rabbit IgG) and a non-specific one (sheep IgG). The analysis using the height histograms reveals many interesting features. We propose a general framework for interpreting these analysis, which enables the discrimination between specific and non-specific complexes.

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AdsorptionAluminum SilicatesAnimalsAntibodiesAntigen-Antibody ComplexAntigen-Antibody ReactionsAntigensBinding Sites, AntibodyGoatsImmunoassayImmunoglobulin GMicroscopy, Atomic ForceSheepJournal Article

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O Ouerghi, A Touhami, A Othmane, H Ben Ouada, C Martelet, C Fretigny, N Jaffrezic-Renault. 2002. Investigating specific antigen/antibody binding with the atomic force microscope.. https://doi.org/10.1016/s1389-0344(02)00046-1

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