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Computational analysis of alternative splicing using EST tissue information.

Hanqing Xie·Wei-yong Zhu·Alon Wasserman·Vladimir Grebinskiy·Andrew Olson·Liat Mintz
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Abstract

Expressed sequence tags (ESTs) from normal and tumor tissues have been deposited in public databases. These ESTs and all mRNA sequences were aligned with the human genome sequence using LEADS, Compugen's alternative splicing modeling platform. We developed a novel computational approach to analyze tissue information of aligned ESTs in order to identify cancer-specific alternative splicing and gene segments highly expressed in particular cancers. Several genes, including one encoding a possible pre-mRNA splicing factor, displayed cancer-specific alternative splicing. In addition, multiple candidate gene segments highly expressed in colon cancers were identified.

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Alternative SplicingComputational BiologyComputer SimulationExpressed Sequence TagsGene LibraryGenome, HumanHumansOrgan SpecificityJournal Article

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Hanqing Xie, Wei-yong Zhu, Alon Wasserman, Vladimir Grebinskiy, Andrew Olson, Liat Mintz. 2002. Computational analysis of alternative splicing using EST tissue information.. https://doi.org/10.1006/geno.2002.6841

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