Development of a frozen cell array as a high-throughput approach for cell-based analysis.

Jean Philippe Stephan; Silvia Schanz; Anne Wong; Peter Schow; Wai Lee T Wong

doi:10.1016/s0002-9440(10)64238-1

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Development of a frozen cell array as a high-throughput approach for cell-based analysis.

Jean Philippe Stephan·Silvia Schanz·Anne Wong·Peter Schow·Wai Lee T Wong

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Abstract

Recent advances in molecular biology, human genetics, and functional genomics tremendously increase the number of molecular targets available for potential therapeutic and diagnostic use. To complement DNA array data, cost-efficient high-throughput technologies providing reliable information at the protein level need to be developed. Here we describe the generation of a frozen cell array that required the use of single cell suspensions and could serve various applications such as the analysis of specific antibody or ligand binding to a large panel of different cell types. As an example, binding of an anti-human epithelial cell adhesion molecule monoclonal antibody to 24 different cell lines has been analyzed using the cell array and compared to the data generated by fluorescence-activated cell sorting. The reliability and flexibility of our frozen cell array technology is compatible with the needs of high-throughput screening for drug discovery and target validation.

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Antibodies Antigens, Neoplasm Cell Adhesion Molecules Cell Line Cryopreservation Cytological Techniques Epithelial Cell Adhesion Molecule Flow Cytometry Humans Sensitivity and Specificity Journal Article

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Jean Philippe Stephan, Silvia Schanz, Anne Wong, Peter Schow, Wai Lee T Wong. 2002. Development of a frozen cell array as a high-throughput approach for cell-based analysis.. https://doi.org/10.1016/s0002-9440(10)64238-1

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