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PubMed · 12223440

Genotype analysis using human hair shaft.

Hsueh-Wei Chang·Ching-Yu Yen·Shyun-Yui Liu·Gad Singer·Ie-Ming Shih
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Abstract

Analysis of genotypes for genetic predisposition of diseases has profound and widespread clinical application and has become a fundamental tool in the study of molecular epidemiology. In this study, we report a convenient and completely noninvasive approach to perform genotyping using terminal cephalic hair shaft. The average and median DNA amounts purified from the hair shaft are 112 +/- 180 (1 SD) pg/mg and 66 pg/mg of hair shaft, respectively. Using hybridization with molecular beacons that can reliably distinguish single nucleotide polymorphisms at different alleles, we are able to analyze the genotypes of APO-E and G-protein beta3 (GNB3) subunit C825T loci in DNA samples obtained from hair shaft in a high throughput format. The amount of DNA obtained from 20 mg of hair shaft is sufficient for multiple independent genotyping assays. These findings demonstrate that terminal hair shaft provides a convenient and reliable genomic DNA source for an analysis of genotypes.

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DNAFemaleGenotypeHairHumansMalePolymerase Chain ReactionPolymorphism, Single NucleotideJournal Article

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Hsueh-Wei Chang, Ching-Yu Yen, Shyun-Yui Liu, Gad Singer, Ie-Ming Shih. 2002. Genotype analysis using human hair shaft.. https://pubmed.ncbi.nlm.nih.gov/12223440/

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