Protease-catalyzed peptide synthesis on solid support.

Rein V Ulijn; Beatriz Baragaña; Peter J Halling; Sabine L Flitsch

doi:10.1021/ja026912d

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Protease-catalyzed peptide synthesis on solid support.

Rein V Ulijn·Beatriz Baragaña·Peter J Halling·Sabine L Flitsch

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Abstract

The direct enzymatic synthesis of peptides from amino acids is widely used as a useful alternative to chemical synthesis. However, good yields of such enzyme-catalyzed reactions require altered reaction conditions to overcome the bias for hydrolysis in aqueous medium. We argue that the synthesis/hydrolysis equilibrium can be shifted toward synthesis in aqueous medium by immobilizing the amine on solid support. In this report, we show the first examples of solid-phase peptide synthesis catalyzed by a protease in bulk aqueous buffer.

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Acrylamides Amino Acids Catalysis Chemistry, Organic Endopeptidases Fluorenes Peptides Polyethylene Glycols Journal Article Research Support, Non-U.S. Gov't

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Rein V Ulijn, Beatriz Baragaña, Peter J Halling, Sabine L Flitsch. 2002-09-18. Protease-catalyzed peptide synthesis on solid support.. https://doi.org/10.1021/ja026912d

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