The use of MassARRAY technology for high throughput genotyping.

Christian Jurinke; Dirk van den Boom; Charles R Cantor; Hubert Köster

doi:10.1007/3-540-45713-5_4

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The use of MassARRAY technology for high throughput genotyping.

Christian Jurinke·Dirk van den Boom·Charles R Cantor·Hubert Köster

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Abstract

This chapter will explore the role of mass spectrometry (MS) as a detection method for genotyping applications and will illustrate how MS evolved from an expert-user-technology to a routine laboratory method in biological sciences. The main focus will be time-of-flight (TOF) based devices and their use for analyzing single-nucleotide-polymorphisms (SNPs, pronounced snips). The first section will describe the evolution of the use of MS in the field of bioanalytical sciences and the protocols used during the early days of bioanalytical MALDI TOF mass spectrometry. The second section will provide an overview on intraspecies sequence diversity and the nature and importance of SNPs for the genomic sciences. This is followed by an exploration of the special and advantageous features of mass spectrometry as the key technology in modern bioanalytical sciences in the third chapter. Finally, the fourth section will describe the MassARRAY technology as an advanced system for automated high-throughput analysis of SNPs.

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Databases, Nucleic Acid Equipment Design Human Genome Project Humans Polymorphism, Restriction Fragment Length Polymorphism, Single Nucleotide Sequence Analysis, DNA Species Specificity Spectrometry, Mass, Electrospray Ionization Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Tandem Repeat Sequences Journal Article Review

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Christian Jurinke, Dirk van den Boom, Charles R Cantor, Hubert Köster. 2002. The use of MassARRAY technology for high throughput genotyping.. https://doi.org/10.1007/3-540-45713-5_4

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