Gene expression profiling using laser capture microdissection.

Randy Todd; Mark W Lingen; Winston P Kuo

doi:10.1586/14737159.2.5.497

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Gene expression profiling using laser capture microdissection.

Randy Todd·Mark W Lingen·Winston P Kuo

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Abstract

Human disease is governed by a complex array of cellular populations and sub-population. Gene expression profiling is proving an important means of understanding and classifying pathophysiologic processes by identifying genes, gene pathways and pathway networks not previously known to be associated with particular diseases. However, disease-associated gene expression can be obscured by surrounding 'normal' tissue. Laser capture microdissection allows gene expression analysis of pooled single cells, cell subpopulations and cell populations. Analysis of laser capture microdissection-procured cells will allow a better understanding of the cellular components of disease.

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DNA, Complementary Dissection Gene Expression Gene Library In Situ Nick-End Labeling Lasers Models, Genetic Molecular Diagnostic Techniques Nucleic Acid Hybridization Oligonucleotide Array Sequence Analysis Polymerase Chain Reaction RNA Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.Review

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Randy Todd, Mark W Lingen, Winston P Kuo. 2002. Gene expression profiling using laser capture microdissection.. https://doi.org/10.1586/14737159.2.5.497

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