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PubMed · 12296819

Spectral imaging fluorescence microscopy.

Tokuko Haraguchi·Takeshi Shimi·Takako Koujin·Noriyo Hashiguchi·Yasushi Hiraoka
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Abstract

The spectral resolution of fluorescence microscope images in living cells is achieved by using a confocal laser scanning microscope equipped with grating optics. This capability of temporal and spectral resolution is especially useful for detecting spectral changes of a fluorescent dye; for example, those associated with fluorescence resonance energy transfer (FRET). Using the spectral imaging fluorescence microscope system, it is also possible to resolve emitted signals from fluorescent dyes that have spectra largely overlapping with each other, such as fluorescein isothiocyanate (FITC) and green fluorescent protein (GFP).

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Fluorescein-5-isothiocyanateFluorescence Resonance Energy TransferFluorescent DyesGreen Fluorescent ProteinsHeLa CellsHumansLuminescent ProteinsMicroscopy, ConfocalMicroscopy, FluorescenceRecombinant Fusion ProteinsJournal ArticleResearch Support, Non-U.S. Gov't

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Tokuko Haraguchi, Takeshi Shimi, Takako Koujin, Noriyo Hashiguchi, Yasushi Hiraoka. 2002. Spectral imaging fluorescence microscopy.. https://doi.org/10.1046/j.1365-2443.2002.00575.x

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