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PubMed · 12351878

Development and characterization of temperature-controlled microreactors for protein crystallization.

Michael Berg·Matthias Urban·Ulrich Dillner·Peter Mühlig·Günter Mayer
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Abstract

Physico-chemical properties of the crystallization of biological macromolecules are of particular interest for an efficient way to get high-quality crystals. Concept and realization of a novel temperature-controlled microreactor to study these parameters is revealed. The characterization of the device is focussed on the temperature distribution across the reaction chamber, its long-term stability and accuracy as well as the regeneration of the surface inside the chamber after contamination with a hydrophilic protein (rGFP). We conclude that a microreactor for in situ observation of a temperature-controlled batch crystallization is in place.

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Chemical PhenomenaChemistry, PhysicalCrystallizationMicrochemistryMicroscopy, ConfocalProteinsTemperatureJournal ArticleResearch Support, Non-U.S. Gov't

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Michael Berg, Matthias Urban, Ulrich Dillner, Peter Mühlig, Günter Mayer. 2002-09-26. Development and characterization of temperature-controlled microreactors for protein crystallization.. https://doi.org/10.1107/s0907444902012726

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