Standard for human thrombin.

I Robertson; P J Gaffney; D R Bangham

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PubMed · 1237945

Standard for human thrombin.

I Robertson·P J Gaffney·D R Bangham

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Abstract

Ten laboratories took part in an international study of the proposed standard for human thrombin. A freeze-dried preparation (coded 70/157) was shown, using clotting time tests, to be suitable to serve as a standard for the assay of human and bovine thrombin preparations. The study shows that assay variation within laboratories was much less than between laboratories. The preparation was stable, having a negligible estimated loss of potency after ten years under the recommended storage conditions (--20 degrees C in the dark). Each ampoule of the standard was assigned a potency of 100 units of thrombin activity, and one unit was defined as the activity contained in 0.0853 mg of the freeze-dried preparation. It was agreed by the participants in the study and by the International Committee on Thrombosis and Haemostasis (Vienna, 1973) that the thrombin preparation coded 70/157 is suitable to serve as an international standard for human thrombin.

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Animals Cattle Drug Stability Drug Storage Fibrinogen Freeze Drying Humans Indicators and Reagents International Cooperation Laboratories Temperature Thrombin United Kingdom Weights and Measures Journal Article

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I Robertson, P J Gaffney, D R Bangham. 1975-09-30. Standard for human thrombin.. https://pubmed.ncbi.nlm.nih.gov/1237945/

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