Transcriptome analysis during cell division in plants.

Peter Breyne; Rozemarijn Dreesen; Klaas Vandepoele; Lieven De Veylder; Frank Van Breusegem; Lindy Callewaert; Stephane Rombauts; Jeroen Raes; Bernard Cannoot; Gilbert Engler; Dirk Inzé; Marc Zabeau

doi:10.1073/pnas.222561199

PubMed · 12393816

Transcriptome analysis during cell division in plants.

Peter Breyne·Rozemarijn Dreesen·Klaas Vandepoele·Lieven De Veylder·Frank Van Breusegem·Lindy Callewaert·Stephane Rombauts·Jeroen Raes·Bernard Cannoot·Gilbert Engler·Dirk Inzé·Marc Zabeau

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Abstract

Using synchronized tobacco Bright Yellow-2 cells and cDNA-amplified fragment length polymorphism-based genomewide expression analysis, we built a comprehensive collection of plant cell cycle-modulated genes. Approximately 1,340 periodically expressed genes were identified, including known cell cycle control genes as well as numerous unique candidate regulatory genes. A number of plant-specific genes were found to be cell cycle modulated. Other transcript tags were derived from unknown plant genes showing homology to cell cycle-regulatory genes of other organisms. Many of the genes encode novel or uncharacterized proteins, indicating that several processes underlying cell division are still largely unknown.

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Cell Cycle Cell Division Cells, Cultured Cytokines Gene Expression Regulation, Plant Genes, Plant Mitosis Polymorphism, Genetic Nicotiana Transcription, Genetic Journal Article Research Support, Non-U.S. Gov't

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Peter Breyne, Rozemarijn Dreesen, Klaas Vandepoele, Lieven De Veylder, Frank Van Breusegem, Lindy Callewaert, Stephane Rombauts, Jeroen Raes, Bernard Cannoot, Gilbert Engler, Dirk Inzé, Marc Zabeau. 2002-10-22. Transcriptome analysis during cell division in plants.. https://doi.org/10.1073/pnas.222561199

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