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Expression and function of phospholipase A(2) in brain.

María A Balboa·Isabel Varela-Nieto·Karin Killermann Lucas·Edward A Dennis
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Abstract

Phospholipase A(2) (PLA(2)) appears to play a fundamental role in cell injury in the central nervous system. We have investigated PLA(2) expression in the astrocytoma cell line 1231N1, and found that GIVA, GIVB, GIVC and GVI PLA(2) messages are expressed. PLA(2) activity is increased by inflammatory/injury stimuli such as interleukin-1beta and lipopolysaccharide in these cells but with very different time courses. The arachidonic acid liberated is converted to prostaglandin E(2), possibly by cyclooxygenase-2, which is induced by inflammatory stimuli. This cell system emerges as a model to study injury/inflammation-related activation of the new PLA(2) forms GIVB and GIVC.

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AnimalsArachidonic AcidsAstrocytomaBrainBrain NeoplasmsCyclooxygenase 2DNA, ComplementaryDinoprostoneDose-Response Relationship, DrugEnzyme InhibitorsHumansInflammationInterleukin-1IsoenzymesLipopolysaccharidesMembrane ProteinsMiceOrganophosphonatesPhospholipases APolymerase Chain ReactionProstaglandin-Endoperoxide SynthasesProtein BindingRatsTumor Cells, CulturedJournal ArticleResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, P.H.S.Review

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María A Balboa, Isabel Varela-Nieto, Karin Killermann Lucas, Edward A Dennis. 2002-10-30. Expression and function of phospholipase A(2) in brain.. https://doi.org/10.1016/s0014-5793(02)03481-6

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