Biotinylated Stealth magnetoliposomes.

Michael Hodenius; Marcel De Cuyper; Linda Desender; Detlef Müller-Schulte; Alois Steigel; Heiko Lueken

doi:10.1016/s0009-3084(02)00105-6

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PubMed · 12426077

Biotinylated Stealth magnetoliposomes.

Michael Hodenius·Marcel De Cuyper·Linda Desender·Detlef Müller-Schulte·Alois Steigel·Heiko Lueken

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Abstract

Dimyristoylphosphatidylethanolamine (DC(14:0)PE) and the dioleoyl analogue (DC(18:1cis)PE) were mixed with alpha-biotinylamido-omega-N-succinimidoxycarbonyl-poly(ethylene glycol) (NHS-PEG-biotin) and quantitatively converted to alpha-biotinylamido-omega-(dimyristoylphosphatidylethanolamino-carbonyl)polyethylene glycol (DC(14:0)PE-PEG-biotin) and the dioleoyl analogue DC(18:1cis)PE-PEG-biotin, respectively. As shown by thin-layer chromatography and 1H NMR spectroscopy, PEGylation of both phosphatidylethanolamine types went to completion if the reaction was performed in organic solvent in the presence of triethylamine. The resulting derivatives were successfully incorporated into both classical phospholipid vesicles and a phospholipid bilayer surrounding nanometer-sized magnetite cores. In the latter case, the so-called activated Stealth(1) magnetoliposomes were produced which very efficiently immobilized streptavidinylated alkaline phosphatase.

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Alkaline Phosphatase Biotin Drug Delivery Systems Ferrosoferric Oxide Iron Liposomes Oxides Polyethylene Glycols Streptavidin Journal Article Research Support, Non-U.S. Gov't

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Michael Hodenius, Marcel De Cuyper, Linda Desender, Detlef Müller-Schulte, Alois Steigel, Heiko Lueken. 2002. Biotinylated Stealth magnetoliposomes.. https://doi.org/10.1016/s0009-3084(02)00105-6

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